Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/131682
Title: Loss of cytochrome P-450 monooxygenase activity and formation of a metabolic-intermediate complex after administration of the neurotoxin MPTP to C57 BL/6J mice
Authors: Shahi, G.S. 
Das, N.P.
Moochhala, S.M.
Issue Date: 1989
Citation: Shahi, G.S., Das, N.P., Moochhala, S.M. (1989). Loss of cytochrome P-450 monooxygenase activity and formation of a metabolic-intermediate complex after administration of the neurotoxin MPTP to C57 BL/6J mice. Asia Pacific Journal of Pharmacology 4 (2) : 89-94. ScholarBank@NUS Repository.
Abstract: The in vivo effects of MPTP on hepatic cytochrome P-450 enzyme activity were studied in relation to the formation of a metabolic-intermediate complex on interaction of MPTP with the hepatic cytochrome P-450 enzyme system in untreated, phenobarbitone (PB)- and β-naphthoflavone (BNF)-pretreated C57BL/6J mice. MPTP depressed cytochrome P-450 monooxygenase activity in general and was found to selectively depress pentoxyresorufin O-dealkylase (PROD) activity in comparison to its effect on ethoxyresorufin O-dealkylase (EROD) activity. MPTP treatment also resulted in a loss of measurable cytochrome P-450 level in non-induced (reduced to 37% of level in non-MPTP treated mice), PB-induced (reduced to 40%) and BNF-induced microsomes (57%). Ferricyanide dissociation studies suggest that in vivo complex formation accounts for some (but not all) of this loss in non-induced (17%) PB-induced (10%) and BNF-induced microsomes (7%). Other possible mechanisms for the loss of cytochrome P-450 which may be involved, such as the conversion of cytochrome P-450 to P-420 and free radical-related damage are also discussed.
Source Title: Asia Pacific Journal of Pharmacology
URI: http://scholarbank.nus.edu.sg/handle/10635/131682
ISSN: 02179687
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

17
checked on Oct 4, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.