Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13160
Title: Investigation of Osteogenic characteristics of human adipose derived stromal cells
Authors: MOHAN CHOTHIRAKOTTU ABRAHAM
Keywords: Adipose derived stromal cells, Mesenchymal stromal cells, ATF4, ATF5, Osteocalcin, Osteogenic differentiation
Issue Date: 18-Jun-2008
Source: MOHAN CHOTHIRAKOTTU ABRAHAM (2008-06-18). Investigation of Osteogenic characteristics of human adipose derived stromal cells. ScholarBank@NUS Repository.
Abstract: Our group showed that activating transcription factor 5 (ATF5) transcript level is downregulated during osteogenic differentiation of ADSC. A close family member of ATF5, ATF4 is an important regulator of osteogenic differentiation in non-osteogenic cell lines. To further understand the role of ATF5 gene, ATF5 was silenced with RNAi and its effect on osteocalcin and ATF4 gene expression were measured with real time PCR. To study whether ATF4 and 5 are binding partners, HEK 293 cells were cotransfected with ATF4 and ATF5 plasmids and visualized with co-immunoprecipitation and immunoblotting.Results: ATF5 silencing was shown to increase the expression of osteocalcin and ATF4 in the majority of donors' ADSC populations. Co-transfection, subsequent co-immunoprecipitation and immunoblotting of cell lysates with ATF4 and ATF5 antibodies demonstrated that ATF4 interact with ATF5 in vivo. Therefore, ATF5 might influence the expression of osteocalcin through binding with ATF4 in stem cells undergoing osteogenic differentiation.
URI: http://scholarbank.nus.edu.sg/handle/10635/13160
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