Please use this identifier to cite or link to this item:
|Title:||Identification of valid housekeeping genes and antioxidant enzyme gene expression change in the aging rat liver|
|Citation:||Chen, J., Rider, D.A., Ruan, R. (2006-01). Identification of valid housekeeping genes and antioxidant enzyme gene expression change in the aging rat liver. Journals of Gerontology - Series A Biological Sciences and Medical Sciences 61 (1) : 20-27. ScholarBank@NUS Repository.|
|Abstract:||Valid housekeeping genes (HKG) are a prerequisite for accurate gene quantification. We performed real-time reverse transcription-polymerase chain reaction to investigate the gene expression of five commonly used HKGs (β-actin, glyceraldehyde-3-phosphate dehydrogenase [GAPDH], ubiquitin C [UBC], hypoxanthine phosphoribosyl-transferase [HPRT], and cyclophilin A [CYPa]) and antioxidant enzymes in the liver of young and old male Fischer rats. A wide variation in HKG expression existed during the aging process, and HPRT was identified as the most stable HKG in rat liver aging. When Cu/Zn-superoxide dismutase gene expression was normalized to HPRT, there was no detectable difference between young and old rats; however, a significant difference was seen when it was normalized to UBC. The variation of UBC caused the misinterpretation of Cu/Zn-superoxide dismutase expression. Catalase expression was significantly decreased, whereas glutathione peroxidase expression was not altered with age. We demonstrated that HPRT was an appropriate HKG, validation of HKGs was vital for accurate quantification, and decreased catalase expression might be involved in the decline of antioxidant defenses during rat liver aging. Copyright 2006 by The Gerontological Society of America.|
|Source Title:||Journals of Gerontology - Series A Biological Sciences and Medical Sciences|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 28, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.