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Title: Pleiotrophin enhances clonal growth and long-term expansion of human embryonic stem cells
Authors: Boon, S.S. 
Chun, M.S.
Vallier, L.
Li, P.
Choong, C.
Boon, H.Y.
Lim, E.H. 
Pedersen, R.A.
Yang, H.H.
Rao, M.
Lima, B.
Keywords: Clonal efficiency and embryonic stem expansion
Feeder cells and human embryonic stem cells
Growth factors and receptors
Transcriptome database
Issue Date: Dec-2007
Source: Boon, S.S., Chun, M.S., Vallier, L., Li, P., Choong, C., Boon, H.Y., Lim, E.H., Pedersen, R.A., Yang, H.H., Rao, M., Lima, B. (2007-12). Pleiotrophin enhances clonal growth and long-term expansion of human embryonic stem cells. Stem Cells 25 (12) : 3029-3037. ScholarBank@NUS Repository.
Abstract: To identify additional growth factors for optimizing propagation of human embryonic stem cells (hESCs), we mined publicly available data sets for the transcriptomes of murine and human ESCs and feeder cells, thereby generating a list of growth factors and complementary receptors. We identified the major pathways previously reported to be important, as well as several new ones. One pathway is the Pleiotrophin (PTN)-Pleiotrophin receptor (PTPRZ1) axis. Murine fibroblasts secrete Ptn, whereas hESCs expressed PTPRZ1, which is downregulated upon differentiation. Depletion of PTPRZ1 resulted in decreased colony formation and lower recovery of hESCs. Supplementation of chemically defined medium for feeder-free propagation of hESCs with PTN allowed higher recovery of hESCs without loss of pluripotency. PTN-PTPRZ1 functions here predominantly via an antiapoptotic effect mediated in part by the activation of Akt. These findings reveal the underlying importance of PTN in hESC survival and its usefulness in the clonal manipulation and large-scale propagation of hESCs. ©AlphaMed Press.
Source Title: Stem Cells
ISSN: 10665099
DOI: 10.1634/stemcells.2007-0372
Appears in Collections:Staff Publications

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