Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/131554
Title: Production of a monoclonal antibody specific to Escherichia coli H33 flagellin by using predetermined polypeptides
Authors: Seah, J.
Kwang, J. 
Keywords: Epitope
Epitope mapping
Escherichia coli
Flagellin
FliC
Serotype
Issue Date: 2002
Citation: Seah, J., Kwang, J. (2002). Production of a monoclonal antibody specific to Escherichia coli H33 flagellin by using predetermined polypeptides. Archives of Microbiology 177 (2) : 192-196. ScholarBank@NUS Repository.
Abstract: The Escherichia coli H serogroup is determined by flagellin, which has both H-type-specific and cross-reactive epitopes. The cross-reactive epitopes are responsible for the cross-reaction found in agglutination. To identify the specific epitope in H33 flagellin, the H33 flagellin gene was sequenced and the encoded central variable region (CVR) was determined. Four overlapping fragments of the CVR were prepared and their specificity was verified using different H-type antisera. Short fragments carrying potential H-type-specific determinants were selected, and monoclonal antibodies (MAbs) against these fragments were prepared. A murine MAb of subtype IgG1 showing specificity to H33 flagellin was produced. The epitope of the MAb was mapped to amino acid residues 250-260.
Source Title: Archives of Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/131554
ISSN: 03028933
Appears in Collections:Staff Publications

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