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|Title:||Localization of linear cytotoxic and pro-apoptotic epitopes in RTX toxin ApxIII of Actinobacillus pleuropneumoniae|
|Citation:||Seah, J.N., Kwang, J. (2004-03-29). Localization of linear cytotoxic and pro-apoptotic epitopes in RTX toxin ApxIII of Actinobacillus pleuropneumoniae. Vaccine 22 (11-12) : 1494-1497. ScholarBank@NUS Repository.|
|Abstract:||RTX toxin ApxIII secreted by Actinobacillus pleuropneumoniae affects porcine pulmonary alveolar macrophages and neutrophils. The distribution of ApxIII linear cytotixic and pro-apoptotic determinants was characterized by neutrophil protection assay, DNA fragmentation and caspase-3 activity using antisera produced against its N-terminus, hydrophobic domain, activation domain, calcium-binding domain and C-terminal half. Neutralization of ApxIII cytotoxicity and pro-apoptotic acitivities was found in antisera raised against its N- and C-termini whereas ApxIII activation domain-specific antiserum expressed cytotoxic-neutralizing activity. No neutralizing activities were detected in antisera against other structural domains of ApxIII suggesting that the putative linear cytotoxic and pro-apoptotic determinants of ApxIII were mapped to its terminal domains and activation domains. © 2003 Elsevier Ltd. All rights reserved.|
|Appears in Collections:||Staff Publications|
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