Please use this identifier to cite or link to this item: https://doi.org/10.1007/s002030000167
Title: Identification of H-specific determinants in flagellin of four Escherichia coli strains
Authors: Seah, J.-N.
Kwang, J. 
Keywords: Escherichia coli
Flagella
Flagellin
FliC
Mapping
Serotype
Issue Date: 2000
Source: Seah, J.-N., Kwang, J. (2000). Identification of H-specific determinants in flagellin of four Escherichia coli strains. Archives of Microbiology 174 (1-2) : 28-34. ScholarBank@NUS Repository. https://doi.org/10.1007/s002030000167
Abstract: The H serogroup of Escherichia coli is determined by the flagellar antigen, flagellin. Sequence analysis of the flagellin gene, fliC, reveals a central variable region and the highly conserved N- and C-termini. This variable region has been shown to encode both H-specific and cross-reactive epitopes. Using polyclonal antibodies, we mapped the linear H-specific determinants in flagellin from four E. coli serotypes O157:H10, O138:H14, O157:H42 and O157:H43. The specificity of all potential fragments was verified with 52 ECRC (Escherichia coli Reference Center) H-specific antisera. Our results indicated that: (a) a specific determinant of H10 flagellin (1263 bp long) maps to the region covering amino acid residues 305-331; (b) a specific determinant of H14 flagellin (1653 bp long) maps to the region covering amino acid residues 430-461; (c) a specific determinant of H42 flagellin (1281 bp long) maps to a region covering amino acid residues 171-201; and (d) a specific determinant of H43 flagellin (1506 bp long) maps to a region covering amino acid residues 200-260.
Source Title: Archives of Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/131543
ISSN: 03028933
DOI: 10.1007/s002030000167
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