Please use this identifier to cite or link to this item:
|Title:||Identification of H-specific determinants in flagellin of four Escherichia coli strains|
|Citation:||Seah, J.-N., Kwang, J. (2000). Identification of H-specific determinants in flagellin of four Escherichia coli strains. Archives of Microbiology 174 (1-2) : 28-34. ScholarBank@NUS Repository. https://doi.org/10.1007/s002030000167|
|Abstract:||The H serogroup of Escherichia coli is determined by the flagellar antigen, flagellin. Sequence analysis of the flagellin gene, fliC, reveals a central variable region and the highly conserved N- and C-termini. This variable region has been shown to encode both H-specific and cross-reactive epitopes. Using polyclonal antibodies, we mapped the linear H-specific determinants in flagellin from four E. coli serotypes O157:H10, O138:H14, O157:H42 and O157:H43. The specificity of all potential fragments was verified with 52 ECRC (Escherichia coli Reference Center) H-specific antisera. Our results indicated that: (a) a specific determinant of H10 flagellin (1263 bp long) maps to the region covering amino acid residues 305-331; (b) a specific determinant of H14 flagellin (1653 bp long) maps to the region covering amino acid residues 430-461; (c) a specific determinant of H42 flagellin (1281 bp long) maps to a region covering amino acid residues 171-201; and (d) a specific determinant of H43 flagellin (1506 bp long) maps to a region covering amino acid residues 200-260.|
|Source Title:||Archives of Microbiology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 20, 2018
WEB OF SCIENCETM
checked on Sep 4, 2018
checked on May 24, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.