Please use this identifier to cite or link to this item:
|Title:||Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture|
|Source:||Nwe, N., He, Q., Damrongwatanapokin, S., Du, Q., Manopo, I., Limlamthong, Y., Fenner, B.J., Spencer, L., Kwang, J. (2006-02-24). Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture. BMC Microbiology 6 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2180-6-16|
|Abstract:||Background: Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine. Results: For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein. Conclusion: Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen. © 2006 Nwe et al; licensee BioMed Central Ltd.|
|Source Title:||BMC Microbiology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 16, 2018
WEB OF SCIENCETM
checked on Dec 13, 2017
checked on Jan 20, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.