Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/131542
Title: Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
Authors: Nwe, N.
He, Q.
Damrongwatanapokin, S.
Du, Q.
Manopo, I.
Limlamthong, Y.
Fenner, B.J.
Spencer, L.
Kwang, J. 
Issue Date: 24-Feb-2006
Citation: Nwe, N., He, Q., Damrongwatanapokin, S., Du, Q., Manopo, I., Limlamthong, Y., Fenner, B.J., Spencer, L., Kwang, J. (2006-02-24). Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture. BMC Microbiology 6 : -. ScholarBank@NUS Repository.
Abstract: Background: Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine. Results: For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 μg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein. Conclusion: Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen. © 2006 Nwe et al; licensee BioMed Central Ltd.
Source Title: BMC Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/131542
ISSN: 14712180
Appears in Collections:Staff Publications

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