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|Title:||Interleukin-18 promoter gene polymorphisms in Chinese patients with systemic lupus erythematosus: Association with CC genotype at position -607|
Single nucleotide polymorphisms
|Citation:||Xu, Q., Soe, K.T., Paramalingam, S.S., Thumboo, J., Koh, D.-R., Fong, K.-Y. (2007-02). Interleukin-18 promoter gene polymorphisms in Chinese patients with systemic lupus erythematosus: Association with CC genotype at position -607. Annals of the Academy of Medicine Singapore 36 (2) : 91-95. ScholarBank@NUS Repository.|
|Abstract:||Introduction: Interleukin-18 (IL-18) is a Th1 cytokine, which is postulated to play a role in systemic lupus erythematosus (SLE). Two single nucleotide polymorphisms (SNPs) in the IL-18 promoter gene region were found to influence the quantitative expression of the IL-18 protein. The aim of this study was to determine whether IL-18 promoter gene polymorphisms are associated with SLE. Materials and Methods: One hundred and thirteen Chinese SLE patients and 218 Chinese healthy individuals were recruited. Genomic DNA was extracted from peripheral venous blood. Sequence-specific primer PCR and restriction fragment length polymorphism (RFLP) analysis were used to genotype the DNA samples for SNP-137 and SNP-607. The following genotypes were obtained: SNP(-607) AA, AC, CC and SNP(-137) GG, GC, CC. Plasma IL-18 concentrations of patients and control subjects were measured by enzyme-linked immunosorbent assay. Results: the frequency of SNP-607/CC genotype was significantly higher in SLE patients (Pc < 0.05) while genotype SNP-607/AC was significantly decreased in SLE patients compared to the control group (Pc|
|Source Title:||Annals of the Academy of Medicine Singapore|
|Appears in Collections:||Staff Publications|
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