Please use this identifier to cite or link to this item:
https://doi.org/10.1021/ja044605x
DC Field | Value | |
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dc.title | Investigating cellular signaling reactions in single attoliter vesicles | |
dc.contributor.author | Pick, H. | |
dc.contributor.author | Schmidt, E.L. | |
dc.contributor.author | Tairi, A.-P. | |
dc.contributor.author | Ilegems, E. | |
dc.contributor.author | Hovius, R. | |
dc.contributor.author | Vogel, H. | |
dc.date.accessioned | 2016-11-28T10:20:18Z | |
dc.date.available | 2016-11-28T10:20:18Z | |
dc.date.issued | 2005-03-09 | |
dc.identifier.citation | Pick, H., Schmidt, E.L., Tairi, A.-P., Ilegems, E., Hovius, R., Vogel, H. (2005-03-09). Investigating cellular signaling reactions in single attoliter vesicles. Journal of the American Chemical Society 127 (9) : 2908-2912. ScholarBank@NUS Repository. https://doi.org/10.1021/ja044605x | |
dc.identifier.issn | 00027863 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/131443 | |
dc.description.abstract | Understanding cellular signaling mediated by cell surface receptors is key to modern biomedical research and drug development. The discovery of a growing number of potential molecular targets and therapeutic compounds requires downscaling and accelerated functional screening. Receptor-mediated cellular responses are typically investigated on single cells or cell populations. Here, we show how to monitor cellular signaling reactions at a yet unreached miniaturization level. On the basis of our observations, cytochalasin induces mammalian cells to extrude from their plasma membrane submicrometer-sized native vesicles. They comprise functional cell surface receptors correctly exposing their extracellular ligand binding sites on the outer vesicle surface and retaining cytosolic proteins in the vesicle interior. As a prototypical example, ligand binding to the ionotropic 5-HT3 receptor and subsequent transmembrane Ca2+ signaling were monitored in single attoliter vesicles. Thus, native vesicles are the smallest autonomous containers capable of performing cellular signaling reactions under physiological conditions. Because a single cell delivers about 50 native vesicles, which can be isolated and addressed as individuals, our concept allows multiple functional analyses of individual cells having a limited availability and opens new vistas for miniaturized bioanalytics. © 2005 American Chemical Society. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/ja044605x | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | MATERIALS SCIENCE | |
dc.description.doi | 10.1021/ja044605x | |
dc.description.sourcetitle | Journal of the American Chemical Society | |
dc.description.volume | 127 | |
dc.description.issue | 9 | |
dc.description.page | 2908-2912 | |
dc.description.coden | JACSA | |
dc.identifier.isiut | 000227479600044 | |
Appears in Collections: | Staff Publications |
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