Please use this identifier to cite or link to this item:
|Title:||Investigating cellular signaling reactions in single attoliter vesicles|
|Citation:||Pick, H., Schmidt, E.L., Tairi, A.-P., Ilegems, E., Hovius, R., Vogel, H. (2005-03-09). Investigating cellular signaling reactions in single attoliter vesicles. Journal of the American Chemical Society 127 (9) : 2908-2912. ScholarBank@NUS Repository. https://doi.org/10.1021/ja044605x|
|Abstract:||Understanding cellular signaling mediated by cell surface receptors is key to modern biomedical research and drug development. The discovery of a growing number of potential molecular targets and therapeutic compounds requires downscaling and accelerated functional screening. Receptor-mediated cellular responses are typically investigated on single cells or cell populations. Here, we show how to monitor cellular signaling reactions at a yet unreached miniaturization level. On the basis of our observations, cytochalasin induces mammalian cells to extrude from their plasma membrane submicrometer-sized native vesicles. They comprise functional cell surface receptors correctly exposing their extracellular ligand binding sites on the outer vesicle surface and retaining cytosolic proteins in the vesicle interior. As a prototypical example, ligand binding to the ionotropic 5-HT3 receptor and subsequent transmembrane Ca2+ signaling were monitored in single attoliter vesicles. Thus, native vesicles are the smallest autonomous containers capable of performing cellular signaling reactions under physiological conditions. Because a single cell delivers about 50 native vesicles, which can be isolated and addressed as individuals, our concept allows multiple functional analyses of individual cells having a limited availability and opens new vistas for miniaturized bioanalytics. © 2005 American Chemical Society.|
|Source Title:||Journal of the American Chemical Society|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 19, 2018
WEB OF SCIENCETM
checked on Sep 10, 2018
checked on May 24, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.