Please use this identifier to cite or link to this item:
|Title:||Host heterogeneous ribonucleoprotein K (hnRNP K) as a potential target to suppress hepatitis B virus replication|
|Citation:||Ng, L.F.P., Chan, M., Chan, S.-H., Cheng, P.C.-P., Leung, E.H.-C., Chen, W.-N., Ren, E.-C. (2005). Host heterogeneous ribonucleoprotein K (hnRNP K) as a potential target to suppress hepatitis B virus replication. PLoS Medicine 2 (7) : 0673-0683. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pmed.0020163|
|Abstract:||Background: Hepatitis B virus (HBV) infection results in complications such as cirrhosis and hepatocellular carcinoma. Suppressing viral replication in chronic HBV carriers is an effective approach to controlling disease progression. Although antiviral compounds are available, we aimed to identify host factors that have a significant effect on viral replication efficiency. Methods and Findings: We studied a group of hepatitis B carriers by associating serum viral load with their respective HBV genomes, and observed a significant association between high patient serum viral load with a natural sequence variant within the HBV enhancer II (Enh II) regulatory region at position 1752. Using a viral fragment as an affinity binding probe, we isolated a host DNA-binding protein belonging to the class of heterogeneous nuclear ribonucleoproteins - hnRNP K - that binds to and modulates the replicative efficiency of HBV. In cell transfection studies, overexpression of hnRNP K augmented HBV replication, while gene silencing of endogenous hnRNP K carried out by small interfering RNAs resulted in a significant reduction of HBV viral load. Conclusion: The evidence presented in this study describes a wider role for hnRNP K beyond maintenance of host cellular functions and may represent a novel target for pharmacologic intervention of HBV replication. © 2005 Ng et al.|
|Source Title:||PLoS Medicine|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jun 17, 2018
WEB OF SCIENCETM
checked on May 16, 2018
checked on Jun 7, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.