Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/131381
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dc.title | Platelet-endothelial cell adhesion molecule-1 gene polymorphism and its soluble level are associated with severe coronary artery stenosis in Chinese Singaporean | |
dc.contributor.author | Wei, H. | |
dc.contributor.author | Fang, L. | |
dc.contributor.author | Chowdhury, S.H. | |
dc.contributor.author | Gong, N. | |
dc.contributor.author | Xiong, Z. | |
dc.contributor.author | Song, J. | |
dc.contributor.author | Mak, K.H. | |
dc.contributor.author | Wu, S. | |
dc.contributor.author | Koay, E. | |
dc.contributor.author | Sethi, S. | |
dc.contributor.author | Lim, Y.L. | |
dc.contributor.author | Chatterjee, S. | |
dc.date.accessioned | 2016-11-28T10:19:32Z | |
dc.date.available | 2016-11-28T10:19:32Z | |
dc.date.issued | 2004-12 | |
dc.identifier.citation | Wei, H., Fang, L., Chowdhury, S.H., Gong, N., Xiong, Z., Song, J., Mak, K.H., Wu, S., Koay, E., Sethi, S., Lim, Y.L., Chatterjee, S. (2004-12). Platelet-endothelial cell adhesion molecule-1 gene polymorphism and its soluble level are associated with severe coronary artery stenosis in Chinese Singaporean. Clinical Biochemistry 37 (12) : 1091-1097. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00099120 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/131381 | |
dc.description.abstract | Platelet-endothelial cell adhesion molecule-1 (PECAM-1) mediates the transendothelial migration of circulating leukocytes, a characteristic change in vascular inflammation leading to atherosclerotic plaque development. We hypothesized that genetic variation and soluble level of PECAM-1 could be associated with coronary artery disease (CAD). We analyzed two single nucleotide polymorphisms (SNPs) of PECAM-1 gene C+373G (Leu125Val) at exon 3, which encodes the first extracellular (Ig)-like domain that mediates the homophilic binding of PECAM-1, and G+1688A (Ser563Asn) at exon 8 in 144 angiographically documented (≥70% stenosis) patients with CAD and 150 age- and sex-matched controls in the Chinese population in Singapore, using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy. Level of plasma soluble PECAM-1 (sPECAM-1) was measured by ELISA. The Leu125Val polymorphism was associated with CAD (P < 0.01). Also, the level of sPECAM-1 is was found to be elevated in CAD patients (P = 0.005). Moreover, subjects with the homozygous GG genotype of the Leu125Val polymorphism had higher sPECAM-1 levels (P = 0.005). The level of sPECAM-1 was further correlated to soluble platelet selectin (sP-selectin, also measured by ELISA), platelet count, and total white blood cell count (WBC), suggesting that platelets are a major source of sPECAM-1 and platelet activation and inflammation may contribute to PECAM-1 elevations in CAD patients. The Leu125Val polymorphism of PECAM-1 and the level of sPECAM-1 are associated with CAD in Chinese in Singapore. The level of sPECAM-1 is also associated with platelet activation and inflammation and correlated to the Leu125Val polymorphism. Our data suggest that PECAM-1 plays an important role in the development of atherosclerosis. © 2004 The Canadian Society of Clinical Chemists. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.clinbiochem.2004.08.006 | |
dc.source | Scopus | |
dc.subject | Atherosclerosis | |
dc.subject | Coronary artery disease | |
dc.subject | Gene polymorphism | |
dc.subject | PECAM-1 | |
dc.type | Article | |
dc.contributor.department | PATHOLOGY | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.sourcetitle | Clinical Biochemistry | |
dc.description.volume | 37 | |
dc.description.issue | 12 | |
dc.description.page | 1091-1097 | |
dc.description.coden | CLBIA | |
dc.identifier.isiut | 000225903000007 | |
Appears in Collections: | Staff Publications |
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