Please use this identifier to cite or link to this item: https://doi.org/10.1017/S0031182001008332
Title: Programmed cell death in a human intestinal parasite, Blastocystis hominis
Authors: Nasirudeen, A.M.A.
Tan, K.S.W. 
Singh, M.
Yap, E.H. 
Keywords: Apoptosis
Blastocystis
Monoclonal antibody.
Programmed cell death
Protozoa
Issue Date: 2001
Source: Nasirudeen, A.M.A., Tan, K.S.W., Singh, M., Yap, E.H. (2001). Programmed cell death in a human intestinal parasite, Blastocystis hominis. Parasitology 123 (3) : 235-246. ScholarBank@NUS Repository. https://doi.org/10.1017/S0031182001008332
Abstract: Although programmed cell death (PCD) has been associated with multicellular organisms, there have been more reports of its presence in some protozoans. Our study shows the existence of PCD in an intestinal protozoan, Blastocystis hominis. Light and electron microscopy, biochemical and flow cytometry studies showed apoptosis-like death in B. hominis cells exposed to a cytotoxic monoclonal antibody (MAb 1DS). B. hominis cells displayed key morphological and biochemical features of apoptosis, namely, nuclear condensation and in situ fragmentation, reduced cytoplasmic volume, some externalization of phosphatidylserine and maintenance of plasma membrane integrity. No oligonucleosomal DNA laddering was observed in gel electrophoresis. This study supports earlier observations that the cellular machinery that is required to carry out PCD may have existed before the advent of multicellularity. Our study also ascribes a novel function for the B. hominis central vacuole in apoptosis; it acts as a repository where apoptotic bodies are stored before being released into the extracellular space.
Source Title: Parasitology
URI: http://scholarbank.nus.edu.sg/handle/10635/131276
ISSN: 00311820
DOI: 10.1017/S0031182001008332
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