Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/131256
Title: Involvement of the heparan sulphate-binding proteins of Helicobacter pylori in its adherence to HeLa S3 and Kato III cell lines
Authors: Guzman-Murillo, M.A.
Ruiz-Bustos, E.
Ho, B. 
Ascencio, F. 
Issue Date: 2001
Source: Guzman-Murillo, M.A., Ruiz-Bustos, E., Ho, B., Ascencio, F. (2001). Involvement of the heparan sulphate-binding proteins of Helicobacter pylori in its adherence to HeLa S3 and Kato III cell lines. Journal of Medical Microbiology 50 (4) : 320-329. ScholarBank@NUS Repository.
Abstract: To determine whether Helicobacter pylori heparan sulphate-binding proteins (HSBPs) are involved in the adherence of H. pylori to HeLa and Kato III cells, monolayers were pre-incubated with various preparations and concentrations of H. pylori HSBPs at 37°C, washed and then challenged with bacteria. HSBPs did not prevent but enhanced H. pylori adherence. However, challenging cultured cells with H. pylori previously incubated with rabbit anti-HSBP IgG resulted in significant inhibition of bacterial adherence. These data demonstrate that the extracellular HSBP plays an important role in promoting H. pylori attachment to Kato III and HeLa S3 cells, that adhesion of H. pylori to Kato III and HeLa S3 cells is promoted by the presence of the 71.5-kDa extracellular HSBP and that rabbit polyclonal antibodies against this HSBP can inhibit adhesion of H. pylori to the cultured cell lines and detach cell-bound H. pylori.
Source Title: Journal of Medical Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/131256
ISSN: 00222615
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

4
checked on Jan 21, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.