Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/131015
Title: Plasmid profiles compared with serotyping and pyocin typing for epidemiological surveillance of Pseudomonas aeruginosa
Authors: Poh, C.L.
Yap, E.H. 
Tay, L.
Bergan, T.
Issue Date: 1988
Source: Poh, C.L., Yap, E.H., Tay, L., Bergan, T. (1988). Plasmid profiles compared with serotyping and pyocin typing for epidemiological surveillance of Pseudomonas aeruginosa. Journal of Medical Microbiology 25 (2) : 109-114. ScholarBank@NUS Repository.
Abstract: The plasmid profiles of 112 clinical isolates of Pseudomonas aeruginosa were determined by two reproducible, rapid, plasmid screening methods. Plasmid DNA was present in 15% of isolates examined. Plasmids varied in size from 1.2 x 106 to 60.2 x 106 mol. wt. The dominant serotypes encountered were O:11 and O:4, which comprised 38% and 12% of strains, respectively. Four pyocin types (1, 10, 3 and 5) dominated (respective frequencies: 56, 15, 12 and 6%). Reproducibility of pyocin typing was distinctly inferior to both plasmid profiling and serotyping. Strains of identical serotypes could be further differentiated by dissimilar plasmid profiles. Serologically untypable or polyagglutinable strains were successfully characterised by plasmid profile patterns. Thus, plasmid profiling was shown to be a useful adjunct to serotyping for the epidemiological typing of P. aeruginosa.
Source Title: Journal of Medical Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/131015
ISSN: 00222615
Appears in Collections:Staff Publications

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