Please use this identifier to cite or link to this item:
|Title:||Human fecal water modifies adhesion of intestinal bacteria to Caco-2 cells|
|Authors:||Lee, Y.-K. |
De Kok, T.M.C.M.
|Citation:||Lee, Y.-K., Hao, W., Ho, P.-S., Nordling, M.M., Low, C.-S., De Kok, T.M.C.M., Rafter, J. (2005). Human fecal water modifies adhesion of intestinal bacteria to Caco-2 cells. Nutrition and Cancer 52 (1) : 35-42. ScholarBank@NUS Repository. https://doi.org/10.1207/s15327914nc5201_5|
|Abstract:||The aqueous phase of feces (fecal water) has been suggested to mediate the effects of diet on colon carcinogenesis. We determined whether human fecal water samples, of varying genotoxic potential, had the capacity to alter adhesion of intestinal bacteria to intestinal (Caco-2) cells. Genotoxicity of fecal water samples was measured using the single-cell gel electrophoresis assay ("comet" assay), and bacterial adhesion was measured using a well-established model system. Fecal water genotoxicity was found to correlate positively with inhibition of adhesion of Escherichia coli strains, Salmonella species, and Enterococcus faecium to Caco-2 cells. The presence of fecal water samples did not interfere with adhesion of Bacteroides and Lactobacillus species. Inhibition of adhesion by fecal water was not due to cytotoxicity to Caco-2 cells as cytotoxicities of most fecal water samples were similar, nor was the inhibitory effect due to bacteriotoxicity as toxicity of fecal waters in the 10 strains of bacteria studied was not detected. Results indicate that components in fecal water may alter adhesion of intestinal bacteria to intestinal cell surfaces and that this effect may be correlated to the genotoxic potential of fecal water. This may have consequences for dietary effects on colon carcinogenesis. Copyright © 2005, Lawrence Erlbaum Associates, Inc.|
|Source Title:||Nutrition and Cancer|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 17, 2018
WEB OF SCIENCETM
checked on Nov 12, 2018
checked on Jul 12, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.