Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF01960881
Title: Genome fingerprinting by pulsed-field gel electrophoresis and ribotyping to differentiate Pseudomonas aeruginosa serotype O11 strains
Authors: Poh, C.L. 
Yeo, C.C.
Tay, L.
Issue Date: Sep-1992
Citation: Poh, C.L., Yeo, C.C., Tay, L. (1992-09). Genome fingerprinting by pulsed-field gel electrophoresis and ribotyping to differentiate Pseudomonas aeruginosa serotype O11 strains. European Journal of Clinical Microbiology & Infectious Diseases 11 (9) : 817-822. ScholarBank@NUS Repository. https://doi.org/10.1007/BF01960881
Abstract: The performance of ribotyping and pulsed-field gel electrophoresis was compared in the differentiation of a collection of 44 Pseudomonas aeruginosa serotype O11 strains isolated in seven hospitals in Singapore. Digestion of genomic DNA by EcoRI and SacI followed by Southern hybridization with the Pseudomonas aeruginosa 16S and 23S rRNA gene revealed seven distinct ribotypes. Ribotyping using a combination of both enzymes revealed 11 ribotypes. In contrast, electrophoretic analysis differentiated 41 different strain types among the 44 clinical isolates using either SpeI or DraI. Pulsed-field gel electrophoresis demonstrated greater sensitivity than ribotyping in the differentiation of Pseudomonas aeruginosa strains of the same ribotype and could thus be used alone in epidemiological investigations of hospital outbreaks of Pseudomonas aeruginosa serotype O11 infection. © 1992 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
Source Title: European Journal of Clinical Microbiology & Infectious Diseases
URI: http://scholarbank.nus.edu.sg/handle/10635/130970
ISSN: 09349723
DOI: 10.1007/BF01960881
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

36
checked on Sep 12, 2018

WEB OF SCIENCETM
Citations

39
checked on Sep 12, 2018

Page view(s)

11
checked on Sep 20, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.