Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13097
Title: Effect of type 1 Collagen and Gelatin on the adhesion and subsequent mineralization of murine pre-osteoblasts
Authors: BAI HUIFEN
Keywords: Atomic force microscopy, collagen type I, gelatin, osteogenic differentiation, focal adhesion, extracellular matrix
Issue Date: 26-Feb-2008
Source: BAI HUIFEN (2008-02-26). Effect of type 1 Collagen and Gelatin on the adhesion and subsequent mineralization of murine pre-osteoblasts. ScholarBank@NUS Repository.
Abstract: Collagen I serves as an organic scaffold in bone and it has been reported that denatured collagen appeared to preserve the osteogenic differentiation potential of mesenchymal stem cells. This study investigated the effect of collagen I and gelatin on the attachment and mineralization of mouse calvaria pre-osteoblasts. Atomic force microscopy (AFM) imaging revealed that the collagen I matrix prepared was D-periodic, while the gelatin obtained upon denaturation exhibited decreased structural integrity. After a contact time of 3 minutes, AFM single-cell force spectroscopy showed that the force required to detach a pre-osteoblast from gelatin was significantly higher than that from collagen I. This is consistent with the finding that more focal adhesions were observed in cells which were plated on gelatin. On gelatin, the pre-osteoblasts also exhibited a higher extent of mineralization upon osteogenic induction. Therefore, compared to collagen I, gelatin appeared to enhance the attachment and subsequent mineralization of pre-osteoblasts.
URI: http://scholarbank.nus.edu.sg/handle/10635/13097
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001 Title Page.pdf8.08 kBAdobe PDF

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002 Preface.pdf10.4 kBAdobe PDF

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003 Acknowledgements.pdf19.77 kBAdobe PDF

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004 Table of Contents.pdf21.2 kBAdobe PDF

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005 Summary.pdf17.14 kBAdobe PDF

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006 Abbreviations.pdf11.37 kBAdobe PDF

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007 List of Figures.pdf20.15 kBAdobe PDF

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008 Chap 1 Introduction.pdf109.46 kBAdobe PDF

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009 Chap 2 Literature Review.pdf1.21 MBAdobe PDF

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010 Chap 3 Materials and Methods.pdf87 kBAdobe PDF

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011 Chap 4 Results.pdf5.95 MBAdobe PDF

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012 Chap 5 Discussion.pdf315.4 kBAdobe PDF

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013 Chap 6 Conclusion.pdf29.59 kBAdobe PDF

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014 Bibliography.pdf43.31 kBAdobe PDF

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