Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/12984
Title: Use of Bordetella pertussis as a live delivery vehicle: Exploring different carrier systems to display an enterovirus 71 vaccine candidate
Authors: HO SI YING
Keywords: Bordetella pertussis, Enterovirus 71, live vaccine, Hand foot and mouth disease, Whooping cough, heterologous antigen carrier
Issue Date: 5-Mar-2008
Source: HO SI YING (2008-03-05). Use of Bordetella pertussis as a live delivery vehicle: Exploring different carrier systems to display an enterovirus 71 vaccine candidate. ScholarBank@NUS Repository.
Abstract: Attenuated Bordetella pertussis represents a promising candidate for nasal delivery of heterologous vaccine antigens. Filamentous hemagglutinin, a major B. pertussis adhesin, has so far been used as the carrier, inducing local and systemic murine immune responses against the heterologous antigen upon nasal administration of the live recombinant bacteria. This project has investigated the B. pertussis autotransporter BrkA as carrier to expose foreign antigens at the bacterial surface. We fused three copies of the neutralizing peptide SP70 from the Enterovirus 71 VP1 capsid protein within the BrkA passenger domain and expressed this in a highly attenuated B. pertussis strain. A recombinant with three copies of SP70 inserted in FHA was also constructed. Both BrkA-(SP70)3 and FHA-(SP70)3 chimera were successfully surface-exposed and secreted respectively. Nasal administration of the former triggered significantly higher murine anti-SP70 neutralizing titers than the latter, clearly indicating BrkAb s potential as a heterologous antigen carrier in attenuated B. pertussis.
URI: http://scholarbank.nus.edu.sg/handle/10635/12984
Appears in Collections:Master's Theses (Open)

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