Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/12968
Title: Functional interactions of Protein Tyrosine Phosphatase Alpha (PTPa) and Src in mouse development and integrin Singaling: Investigation of double PTPa/Src-deficient mice and cells
Authors: CHEN MIN
Keywords: Protein tyrosine phosphatase alpha; SFKs; integrin signaling; embryonic development; cell adhesion and spreading; cytoskeletal organization
Issue Date: 18-Dec-2007
Source: CHEN MIN (2007-12-18). Functional interactions of Protein Tyrosine Phosphatase Alpha (PTPa) and Src in mouse development and integrin Singaling: Investigation of double PTPa/Src-deficient mice and cells. ScholarBank@NUS Repository.
Abstract: The major cellular targets of protein tyrosine phosphatase alpha (PTPa) are the Src family kinases (SFKs), including Src and Fyn. To investigate the roles and signaling interactions of PTPa and SFKs, genetically modified mice were generated with a combined ablation of PTPa and Src (PTPa-/-Src-/-). Double mutant mice were viable, lacked detectable unique developmental defects, but exhibited higher postnatal mortality than Src-null mice. PTPa and SFKs are components of the integrin signaling pathway. At a cellular level, fibroblasts from PTPa-/-Src-/- mouse embryos displayed particularly severe defects in integrin-stimulated cell processes. At the molecular level, a sustained Erk activation was detected in the PTPa-/-Src-/- cells, indicating a negative role for PTPa-Src in integrin signaling events. Additionally, PTPa underwent integrin-stimulated tyrosine phosphorylation that was mediated by Src and other SFKs. This was found to be required for a novel second role of PTPa in cell processes leading to migration.
URI: http://scholarbank.nus.edu.sg/handle/10635/12968
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