Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1012766116568
Title: Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects
Authors: Wong, V. 
Williams, D.
Colby, C.B.
Keywords: Couette viscometer
Mathematical simulation
Oxygen depletion
Plant cell culture
Shear sensitivity
Issue Date: 2001
Citation: Wong, V., Williams, D., Colby, C.B. (2001). Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects. Biotechnology Letters 23 (22) : 1841-1847. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1012766116568
Abstract: Mathematical simulation and experimental measurement of dissolved O2 were performed for extended (up to 8 h) shear testing of Daucus carota (carrot) cell cultures in a conventional Couette viscometer (0.625 mm annulus). The results suggest O2 depletion below critical levels for cell growth may occur. A novel design modification incorporating an O2-permeable silicone-layer spun cast on a porous ceramic bowl was devised. It significantly improved oxygenation of the cell cultures, keeping dissolved O2 near saturation.
Source Title: Biotechnology Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/129636
ISSN: 01415492
DOI: 10.1023/A:1012766116568
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