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|Title:||Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects|
|Authors:||Wong, V. |
Plant cell culture
|Citation:||Wong, V., Williams, D., Colby, C.B. (2001). Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects. Biotechnology Letters 23 (22) : 1841-1847. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1012766116568|
|Abstract:||Mathematical simulation and experimental measurement of dissolved O2 were performed for extended (up to 8 h) shear testing of Daucus carota (carrot) cell cultures in a conventional Couette viscometer (0.625 mm annulus). The results suggest O2 depletion below critical levels for cell growth may occur. A novel design modification incorporating an O2-permeable silicone-layer spun cast on a porous ceramic bowl was devised. It significantly improved oxygenation of the cell cultures, keeping dissolved O2 near saturation.|
|Source Title:||Biotechnology Letters|
|Appears in Collections:||Staff Publications|
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