Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/129558
Title: Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus
Authors: Fielding, B.C.
Tan, Y.-J. 
Shuo, S. 
Tan, T.H.P.
Ooi, E.-E. 
Lim, S.G. 
Hong, W. 
Goh, P.-Y. 
Issue Date: Jul-2004
Citation: Fielding, B.C., Tan, Y.-J., Shuo, S., Tan, T.H.P., Ooi, E.-E., Lim, S.G., Hong, W., Goh, P.-Y. (2004-07). Characterization of a unique group-specific protein (U122) of the severe acute respiratory syndrome coronavirus. Journal of Virology 78 (14) : 7311-7318. ScholarBank@NUS Repository.
Abstract: A novel coronavirus (CoV) has been identified as the etiological agent of severe acute respiratory syndrome (SARS). The SARS-CoV genome encodes the characteristic essential CoV replication and structural proteins. Additionally, the genome contains six group-specific open reading frames (ORFs) larger than 50 amino acids, with no known homologues. As with the group-specific genes of the other CoVs, little is known about the SARS-CoV group-specific genes. SARS-CoV ORF7a encodes a putative unique 122-amino-acid protein, designated U122 in this study. The deduced sequence contains a probable cleaved signal sequence and a C-terminal transmembrane helix, indicating that U122 is likely to be a type I membrane protein. The C-terminal tail also contains a typical endoplasmic reticulum (ER) retrieval motif, KRKTE. U122 was expressed in SARS-CoV-infected Vero E6 cells, as it could be detected by Western blot and immunofluorescence analyses. U122 is localized to the perinuclear region of both SARS-CoV-infected and transfected cells and colocalized with ER and intermediate compartment markers. Mutational analyses showed that both the signal peptide sequence and ER retrieval motif were functional.
Source Title: Journal of Virology
URI: http://scholarbank.nus.edu.sg/handle/10635/129558
ISSN: 0022538X
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

45
checked on May 11, 2018

WEB OF SCIENCETM
Citations

45
checked on May 16, 2018

Page view(s)

13
checked on May 17, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.