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|Title:||2-[18F]-2-deoxy-D-Glucose (FDG) uptake in human tumor cells is related to the expression of GLUT-1 and hexokinase II|
|Citation:||Ong, L.-C., Jin, Y., Song, I.-C., Yu, S., Zhang, K., Chow, P.K.H. (2008). 2-[18F]-2-deoxy-D-Glucose (FDG) uptake in human tumor cells is related to the expression of GLUT-1 and hexokinase II. Acta Radiologica 49 (10) : 1145-1153. ScholarBank@NUS Repository. https://doi.org/10.1080/02841850802482486|
|Abstract:||Background: The uptake of 2-[18F]-2-deoxy-D-glucose (18F-FDG) is widely used as a marker of increased glucose metabolism to monitor progression of cancers with positron emission tomography (PET). Many tumors have been shown to overexpress facilitated glucose transporters, especially GLUT-1 and a glycolytic enzyme, hexokinase II. Purpose: To define whether a quantitative relationship exists between the expression levels of GLUT-1 and hexokinase II, and 18F-FDG uptake in human cancer xenografts. Material and Methods: We determined the expression levels of both GLUT-1 and hexokinase II in normal cells and in five different human cancer cell lines (AGS, A431, A549, Colo 320 HSR, and HepG2) using Western blot analysis. In vitro assays of 18F-FDG uptake in cultures were performed, and subsequently representative cell lines were inoculated onto the flanks of severe combined immunodeficient (SCID) mice. To establish an orthotopic model of human hepatocellular carcinoma (HCC), cells were injected into the intraportal vein of SCID mice. 18F-FDG uptake in vivo was assessed by subjecting mice to PET imaging. Results: All cell lines were shown to express higher amounts of GLUT-1 and hexokinase II compared with fibroblast controls. Our results from in vitro 18F-FDG uptake assays also correlated with the Western blot results. All xenografts gave highly positive results at microPET imaging, and a strong correlation (R 2=0.88, P|
|Source Title:||Acta Radiologica|
|Appears in Collections:||Staff Publications|
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