Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/12930
DC Field | Value | |
---|---|---|
dc.title | A comprehensive proteome analysis of hepatitis B virus- associated hepatocellular carcinoma | |
dc.contributor.author | ZUBAIDAH BINTE MOHAMED RAMDZAN | |
dc.date.accessioned | 2010-04-08T10:28:31Z | |
dc.date.available | 2010-04-08T10:28:31Z | |
dc.date.issued | 2009-08-24 | |
dc.identifier.citation | ZUBAIDAH BINTE MOHAMED RAMDZAN (2009-08-24). A comprehensive proteome analysis of hepatitis B virus- associated hepatocellular carcinoma. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/12930 | |
dc.description.abstract | Hepatocellular carcinoma (HCC), one of the major cancer causing deaths, is commonly attributed to persistent viral infection. To date, the molecular pathogenesis of HCC remains elusive. In this study, proteomic based approaches were adopted to unravel the molecular players involved in HCC. Three quantitative approaches using 2-D DIGE, as well as cICAT and iTRAQ labels coupled with 2-DLC were concurrently used to analyze tumours from moderately- and poorly-differentiated HBV-related HCC. Data obtained from this study enabled identification of possible HCC molecular players. In addition to protein verifications, further analysis was conducted on a novel protein family, far upstream binding proteins (FUBPs). The over-expression of FUBPs in both stages of HCC is of particular interest due to their transcriptional activity on proto-oncogene, c-Myc. c-Myc plays an important role in HCC progression, especially in a viral associated carcinogenesis. It was also observed to be elevated in the tissues used in this study. Exact activators and functions of c-Myc, however remain poorly understood. Preliminary work using siRNA transfections on liver cell lines, proposes FUBPs as a possible upstream player that are involved in HBV-related HCC tumorigenesis. | |
dc.language.iso | en | |
dc.subject | Hepatocellular carcinoma (HCC), HBV, 2-D DIGE, cICAT, iTRAQ, Far Upstream Binding Protein (FUBP) | |
dc.type | Thesis | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.supervisor | CHUNG CHING MING, MAXEY | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
ZubaidahRM.pdf | 13.83 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.