Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/12923
Title: Characterizing Helicobacter pylori biofilm as a survival strategy in the extragastric environment
Authors: NG CHOW GOON
Keywords: Helicobacter pylori, biofilm, interleukin-8, 16S rRNA, cytotoxin-associated gene A, AGS cells
Issue Date: 17-Jun-2009
Source: NG CHOW GOON (2009-06-17). Characterizing Helicobacter pylori biofilm as a survival strategy in the extragastric environment. ScholarBank@NUS Repository.
Abstract: Bacteria form biofilm to overcome environmental stress. This study shows that Helicobacter pylori forms biofilm optimally in low nutrient and mild acidic conditions and its biofilm-forming ability is strain- dependent. Interestingly, biofilm was found to have higher cytotoxin-associated gene A (CagA), a H. pylori virulence factor, and induce higher IL-8 production in AGS gastric cells than its planktonic counterpart. Together with significant reduction of biofilm formation (P<0.05) by cagA-isogenic mutant, these imply that CagA may play a role in biofilm formation. Furthermore, the detection of 16S rRNA in drinking water and the water-biofilms indicates the probable association of H. pylori with biofilm in the extragastric environment. The study also showed that H. pylori can develop biofilm in milk thereby suggesting that H. pylori-contaminated milk may also be a potential transmission route. Therefore, this study highlights the probable role of biofilm in the transmission of H. pylori in the extragastric environments.
URI: http://scholarbank.nus.edu.sg/handle/10635/12923
Appears in Collections:Master's Theses (Open)

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01 Summary Introduction and literature survey_Ng Chow Goon.pdf2.98 MBAdobe PDF

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02 Table of content and publications_Ng Chow Goon.pdf63.25 kBAdobe PDF

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03 Materials and methods_Ng Chow Goon.pdf278.42 kBAdobe PDF

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04 Results_Ng Chow Goon.pdf29.72 MBAdobe PDF

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05 Discussion_Ng Chow Goon.pdf191.61 kBAdobe PDF

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06 References_Ng Chow Goon.pdf195.34 kBAdobe PDF

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07 Appendices_Ng Chow Goon.pdf233.18 kBAdobe PDF

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