Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/129142
Title: GENOME-SCALE STRUCTUROMICS FOR THE DISCOVERY OF FUNCTIONAL TRANSCRIPT DOMAINS
Authors: NICHOLAS JAMES BOGARD
Keywords: NGS, RNA, structure, riboswitch, PARS, intron
Issue Date: 21-Apr-2015
Citation: NICHOLAS JAMES BOGARD (2015-04-21). GENOME-SCALE STRUCTUROMICS FOR THE DISCOVERY OF FUNCTIONAL TRANSCRIPT DOMAINS. ScholarBank@NUS Repository.
Abstract: We have adapted the Parallel Analysis of RNA Structure (PARS) method to probe for functional transcripts across both prokaryotic and eukaryotic genomes. We used an unbiased approach of transcribing all regions of the genome, in vitro, into equally sized short RNAs that were then treated with single- or double-strand RNA endonucleases. Our method allowed us to generate a more evenly distributed pool of transcripts that includes sequences that are typically underrepresented or lost during RNA processing. We detected strong signatures of structure across mRNA as well as new, potentially functional, loci associated with stable RNA conformations. We further applied the method to characterize the RNA conformational landscape in vitamin-enriched environments.
URI: http://scholarbank.nus.edu.sg/handle/10635/129142
Appears in Collections:Ph.D Theses (Open)

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