Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/12914
Title: Inactivation and repair of escherichia coli following UV disinfection: Influencing factors and photolyase activity
Authors: QUEK PUAY HOON, ELAINE
Keywords: DNA repair, dark repair, Escherichia coli, photolyase, photoreactivation, UV disinfection
Issue Date: 19-Nov-2008
Source: QUEK PUAY HOON, ELAINE (2008-11-19). Inactivation and repair of escherichia coli following UV disinfection: Influencing factors and photolyase activity. ScholarBank@NUS Repository.
Abstract: DNA repair following UV disinfection of drinking water is a serious concern because it can lead to deterioration in the microbiological quality of the water. There is therefore a need to examine DNA repair processes closely. In this thesis, photoreactivation and dark repair of Escherichia coli was investigated and compared to that of the pathogenic E. coli O157:H7, and indicators for the two processes were identified. DNA repair was also examined under various environmental conditions such as photoreactivating light intensity with fluorescent and sunlight and incubation temperature, as well as operating conditions such as UV lamp type and UV doses. A focused investigation on the effect of UV disinfection on photoreactivation was also carried out by looking at photolyase enzyme, and its ability to perform dimer repair under varying operating disinfection conditions (disinfection lamp type, UV dose, and UV wavelength used).
URI: http://scholarbank.nus.edu.sg/handle/10635/12914
Appears in Collections:Ph.D Theses (Open)

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