Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/129124
Title: FAST RAMAN SPECTROSCOPY AND STIMULATED RAMAN SCATTERING MICROSCOPY FOR LABEL-FREE BIOMOLECULAR IMAGING
Authors: WANG ZI
Keywords: coherent Raman scattering, line scan, nonlinear optical imaging, stimulated Raman scattering, TPEF, SHG
Issue Date: 31-Mar-2016
Citation: WANG ZI (2016-03-31). FAST RAMAN SPECTROSCOPY AND STIMULATED RAMAN SCATTERING MICROSCOPY FOR LABEL-FREE BIOMOLECULAR IMAGING. ScholarBank@NUS Repository.
Abstract: We develop new methods in this thesis work by employing the parallel excitation and detection schemes to boost the Raman imaging speed. We first report the development of a line-scan Raman technique for rapid Raman imaging, which can acquire Raman image of 200 × 200 pixels within 3.3 minutes. We also develop a line-scan stimulated Raman scattering (SRS) imaging technique for video-rate biochemical imaging with 5 times lower excitation power. The developed line-scan SRS imaging technique was based on a linear detector of large full well capacity controlled by a field programmable gate array (FPGA) to achieve video-rate SRS imaging with high quality and low excitation power. Further, we also develop a polarization-resolved hyperspectral SRS imaging technique to visualize the biochemical distributions and biomolecular orientations of unstained cells and tissue (e.g., tooth, colonic tissue, live gastric cells).
URI: http://scholarbank.nus.edu.sg/handle/10635/129124
Appears in Collections:Ph.D Theses (Open)

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