Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/12902
Title: Proteomics analysis of Pro-inflammatory Cytokine-stimulated human lung fibroblasts and bronchial epithelial cells
Authors: LIAO WUPENG
Keywords: TNF-alpha, IFN-gamma, airway resident cells, proteomics, DCIP, apoL2
Issue Date: 5-Nov-2008
Source: LIAO WUPENG (2008-11-05). Proteomics analysis of Pro-inflammatory Cytokine-stimulated human lung fibroblasts and bronchial epithelial cells. ScholarBank@NUS Repository.
Abstract: Airway resident cells like epithelia and fibroblasts are critical regulators of airway immunity, inflammation and remodeling. The present study investigated global protein profilings of normal human bronchial epithelial cells (NHBE) and normal human lung fibroblasts (NHLF) stimulated with pro-inflammatory cytokines tumor necrosis factor (TNF)-alpha and/or interferon (IFN)-gamma. Total proteins from cell lysates and culture media were separated by 2-dimensional gel electrophoresis (2-DE), and differentially expressed proteins were identified by MALDI-TOF MS and MS/MS. Dendritic cell-derived IFN-gamma-induced protein (DCIP) was found up-regulated by TNF-alpha in lung fibroblasts for the first time, which mediated TNF-o ! stimulation of human lung fibroblasts. In addition, apolipoprotein L2 (apoL2) was found for the first time ever to be increased by IFN-gamma in epithelial cells, which in turn affected IFN-gamma regulation of bronchial epithelial cell proliferation. These studies reveal that proteomics is an efficient way to identify novel cytokine-regulated proteins and characterize their relevant biological actions.
URI: http://scholarbank.nus.edu.sg/handle/10635/12902
Appears in Collections:Ph.D Theses (Open)

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