Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/12870
Title: Functional genomic analysis of fission yeast protein kinases and characterisation of a novel kinase, Pdk1p involved in mitosis and cytokinesis
Authors: BIMBO ANDREA
Keywords: Schizosaccharomyces pombe, fission yeast, protein kinase, Pdk1p, mitosis, cytokinesis
Issue Date: 23-Mar-2007
Source: BIMBO ANDREA (2007-03-23). Functional genomic analysis of fission yeast protein kinases and characterisation of a novel kinase, Pdk1p involved in mitosis and cytokinesis. ScholarBank@NUS Repository.
Abstract: In order to discover novel proteins involved in mitosis and cytokinesis, the genome-wide bank of fission yeast mutants deleted for open reading frames encoding protein kinases was generated. Systematic analysis of deletion mutants revealed that 46% of the 89 strains were hypersensitive to at least one of the 17 stress factors tested. Furthermore, the capability of deletion mutants to undergo mating, meiosis and sporulation was examined. By screening this bank of mutants, a novel protein homologous to phosphoinositide dependent protein kinases, Pdk1p, was identified. Pdk1p is not essential for the survival of the cells, but its deletion results in several mitotic and cytokinetic defects. Characterization of Pdk1p revealed that it plays important role in proper segregation of chromosomes as well as placement and timely assembly of the actomyosin ring. These functions are likely accomplished by contributing to processes regulated by the fission yeast homologue of polo like kinases, Plo1p. The effects of Pdk1p on cytokinesis in part are mediated through Mid1p and the septation initiation network.
URI: http://scholarbank.nus.edu.sg/handle/10635/12870
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