Please use this identifier to cite or link to this item: https://doi.org/10.1186/1756-6606-4-1
Title: Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation
Authors: Je, H.S. 
Ji, Y.
Wang, Y.
Yang, F.
Wu, W.
Lu, B.
Issue Date: 2011
Citation: Je, H.S., Ji, Y., Wang, Y., Yang, F., Wu, W., Lu, B. (2011). Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation. Molecular Brain 4 (1) : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1756-6606-4-1
Abstract: Background. Neurotrophins elicit both acute and long-term modulation of synaptic transmission and plasticity. Previously, we demonstrated that the long-term synaptic modulation requires the endocytosis of neurotrophin-receptor complex, the activation of PI3K and Akt, and mTOR mediated protein synthesis. However, it is unclear whether the long-term synaptic modulation by neurotrophins depends on protein synthesis in pre- or post-synaptic cells. Results. Here we have developed an inducible protein translation blocker, in which the kinase domain of protein kinase R (PKR) is fused with bacterial gyrase B domain (GyrB-PKR), which could be dimerized upon treatment with a cell permeable drug, coumermycin. By genetically targeting GyrB-PKR to specific cell types, we show that NT-3 induced long-term synaptic modulation requires presynaptic, but not postsynaptic protein synthesis. Conclusions. Our results provide mechanistic insights into the cell-specific requirement for protein synthesis in the long-term synaptic modulation by neurotrophins. The GyrB-PKR system may be useful tool to study protein synthesis in a cell-specific manner. © 2011 Je et al; licensee BioMed Central Ltd.
Source Title: Molecular Brain
URI: http://scholarbank.nus.edu.sg/handle/10635/128617
ISSN: 17566606
DOI: 10.1186/1756-6606-4-1
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