Please use this identifier to cite or link to this item: https://doi.org/10.1039/c0an00689k
Title: Evaluation of apparent non-specific protein loss due to adsorption on sample tube surfaces and/or altered immunogenicity
Authors: Dixit, C.K.
Vashist, S.K. 
MacCraith, B.D.
O'Kennedy, R.
Issue Date: 7-Apr-2011
Citation: Dixit, C.K., Vashist, S.K., MacCraith, B.D., O'Kennedy, R. (2011-04-07). Evaluation of apparent non-specific protein loss due to adsorption on sample tube surfaces and/or altered immunogenicity. Analyst 136 (7) : 1406-1411. ScholarBank@NUS Repository. https://doi.org/10.1039/c0an00689k
Abstract: The non-specific loss of protein analytes can have a major effect on assay results particularly where the concentrations of such analytes are extremely low and the matrix is complex. This report assesses how the protein incubated in sample tubes may be lost due to adsorption. Use of proteins, such as bovine serum albumin (BSA), may be used to pre-treat tubes to reduce such losses. However, such losses may also be associated with structural perturbations leading to changes in immunogenicity (as a result of alterations in specific epitope-related conformations). This can lead to erroneous results or lack of comparability with a range of methodologies such as the bicinchoninic protein assay and immunoassays or when surface plasmon resonance (SPR)-based approaches are used. A model system to evaluate these phenomena is proposed. © The Royal Society of Chemistry 2011.
Source Title: Analyst
URI: http://scholarbank.nus.edu.sg/handle/10635/128550
ISSN: 00032654
DOI: 10.1039/c0an00689k
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