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|Title:||Supported membranes embedded with fixed arrays of gold nanoparticles|
|Citation:||Lohmüller, T., Triffo, S., O'Donoghue, G.P., Xu, Q., Coyle, M.P., Groves, J.T. (2011-11-09). Supported membranes embedded with fixed arrays of gold nanoparticles. Nano Letters 11 (11) : 4912-4918. ScholarBank@NUS Repository. https://doi.org/10.1021/nl202847t|
|Abstract:||We present a supported membrane platform consisting of a fluid lipid bilayer membrane embedded with a fixed array of gold nanoparticles. The system is realized by preforming a hexagonal array of gold nanoparticles (∼5-7 nm) with controlled spacing (∼50-150 nm) fixed to a silica or glass substrate by block copolymer lithography. Subsequently, a supported membrane is assembled over the intervening bare substrate. Proteins or other ligands can be associated with the fluid lipid component, the fixed nanoparticle component, or both, providing a hybrid interface consisting of mobile and immobile components with controlled geometry. We test different biochemical coupling strategies to bind individual proteins to the particles surrounded by a fluid lipid membrane. The coupling efficiency to nanoparticles and the influence of nanoparticle arrays on the surrounding membrane integrity are characterized by fluorescence imaging, correlation spectroscopy, and super-resolution fluorescence microscopy. Finally, the functionality of this system for live cell experiments is tested using the ephrin-A1-EphA2 juxtacrine signaling interaction in human breast epithelial cells. © 2011 American Chemical Society.|
|Source Title:||Nano Letters|
|Appears in Collections:||Staff Publications|
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