Please use this identifier to cite or link to this item:
|Title:||Myosin IIA modulates T cell receptor transport and CasL phosphorylation during early immunological synapse formation|
|Citation:||Yu, Y., Fay, N.C., Smoligovets, A.A., Wu, H.-J., Groves, J.T. (2012-02-08). Myosin IIA modulates T cell receptor transport and CasL phosphorylation during early immunological synapse formation. PLoS ONE 7 (2) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0030704|
|Abstract:||Activation of T cell receptor (TCR) by antigens occurs in concert with an elaborate multi-scale spatial reorganization of proteins at the immunological synapse, the junction between a T cell and an antigen-presenting cell (APC). The directed movement of molecules, which intrinsically requires physical forces, is known to modulate biochemical signaling. It remains unclear, however, if mechanical forces exert any direct influence on the signaling cascades. We use T cells from AND transgenic mice expressing TCRs specific to the moth cytochrome c 88-103 peptide, and replace the APC with a synthetic supported lipid membrane. Through a series of high spatiotemporal molecular tracking studies in live T cells, we demonstrate that the molecular motor, non-muscle myosin IIA, transiently drives TCR transport during the first one to two minutes of immunological synapse formation. Myosin inhibition reduces calcium influx and colocalization of active ZAP-70 (zeta-chain associated protein kinase 70) with TCR, revealing an influence on signaling activity. More tellingly, its inhibition also significantly reduces phosphorylation of the mechanosensing protein CasL (Crk-associated substrate the lymphocyte type), raising the possibility of a direct mechanical mechanism of signal modulation involving CasL. © 2012 Yu et al.|
|Source Title:||PLoS ONE|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 10, 2018
WEB OF SCIENCETM
checked on May 30, 2018
checked on Mar 11, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.