Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/128409
Title: LIVE IMAGING OF OSTEOBLAST-OSTEOCLAST COUPLING IN OSX DEFICIENT AND BISPHOSPHONATE TREATED MEDAKA FISH
Authors: YU TINGSHENG
Keywords: osteoblast,osteoclast,osterix,bisphosphonate,medaka,live imaging
Issue Date: 28-Jun-2016
Citation: YU TINGSHENG (2016-06-28). LIVE IMAGING OF OSTEOBLAST-OSTEOCLAST COUPLING IN OSX DEFICIENT AND BISPHOSPHONATE TREATED MEDAKA FISH. ScholarBank@NUS Repository.
Abstract: In mammals, the transcription factor, Osterix (Osx) controls osteoblast differentiation. Its role in non-mammalian bone formation, however, remains elusive. In the first project, osx knock-out medaka mutants were generated and characterized. This showed a crucial role for osx in osteoblast maturation and skeletogenesis in a teleost, and revealed conserved and non-conserved features of osx function during vertebrate bone formation. Osteoblasts interact with osteoclasts to achieve skeletal homeostasis. Excess osteoclast activity leads to diseases such as osteoporosis. In the second project, transgenic medaka larvae with osteoporosis-like lesions were treated with bisphosphonates, compounds commonly used in human therapy. Live imaging in transgenic medaka reporter lines demonstrated the anti-resorptive activity of these compounds in vivo and allowed the visualization of bone cell behaviour at various stages during bone resorption and repair. This establishes medaka as a model for drug screening of bone modulating compounds and allows an in vivo analysis of osteoblast-osteoclast interactions.
URI: http://scholarbank.nus.edu.sg/handle/10635/128409
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