Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/128403
Title: MOLECULAR BIOLOGY OF AQUAPORINS, RHESUS GLYCOPROTEINS AND UREA TRANSPORTERS IN AFRICAN LUNGFISH, PROTOPTERUS ANNECTENS
Authors: CHNG YOU RONG
Keywords: Aqp, Rhgp, Ut, aestivation, ammonia exposure, differential gill transcriptome
Issue Date: 5-May-2016
Citation: CHNG YOU RONG (2016-05-05). MOLECULAR BIOLOGY OF AQUAPORINS, RHESUS GLYCOPROTEINS AND UREA TRANSPORTERS IN AFRICAN LUNGFISH, PROTOPTERUS ANNECTENS. ScholarBank@NUS Repository.
Abstract: African lungfishes can undergo aestivation during drought and tolerate exposure to 100 mmol l−1 NH4Cl. In fishes, transepithelial water transport involves aquaporins (Aqp), while ammonia and urea transport involves rhesus glycoproteins (Rhgp) and urea transporters (Ut), respectively. In this study, four aqp (aqp1aa, aqp3a, aqp8aa, aqp11), three rhgp (rhag, rhbg, rhcg) and three ut (ut-a2a, ut-a2b, ut-a2c) isoforms were identified. Aestivation and ammonia exposure led to changes in the expression of aqp/Aqp, rhgp/Rhgp and ut/Ut in the gills, skin and liver of P. annectens. Furthermore, differential gill transcriptomic analyses during the three phases of aestivation confirmed that increased transcription could be extended to other genes during the maintenance phase. There are up- and down-regulation of genes during the induction and arousal phases, confirming the importance of structural reconstruction, and offered insights into how the physiological functions of the gills were switched on and off, during these two phases of aestivation.
URI: http://scholarbank.nus.edu.sg/handle/10635/128403
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