Please use this identifier to cite or link to this item:
|Title:||Protective role of adenylate cyclase in the context of a live pertussis vaccine candidate|
|Authors:||Lim, A. |
|Citation:||Lim, A., Ng, J.K.W., Locht, C., Alonso, S. (2014-01). Protective role of adenylate cyclase in the context of a live pertussis vaccine candidate. Microbes and Infection 16 (1) : 51-60. ScholarBank@NUS Repository. https://doi.org/10.1016/j.micinf.2013.10.002|
|Abstract:||Despite high vaccination coverage, pertussis remains an important respiratory infectious disease and the least-controlled vaccine-preventable infectious disease in children. Natural infection with Bordetella pertussis is known to induce strong and long-lasting immunity that wanes later than vaccine-mediated immunity. Therefore, a live attenuated B. pertussis vaccine, named BPZE1, has been developed and has recently completed a phase I clinical trial in adult human volunteers. In this study, we investigated the contribution of adenylate cyclase (CyaA) in BPZE1-mediated protection against pertussis. A CyaA-deficient BPZE1 mutant was thus constructed. Absence of CyaA did not compromise the adherence properties of the bacteria onto mammalian cells. However, the CyaA-deficient mutant displayed a slight impairment in the ability to survive within macrophages compared to the parental BPZE1 strain. Invivo, whereas the protective efficacy of the CyaA-deficient mutant was comparable to the parental strain at a vaccine dose of 5×105colony forming units (CFU), it was significantly impaired at a vaccine dose of 5×103CFU. This impairment correlated with impaired lung colonization ability, and impaired IFN-γ production in the animal immunized with the CyaA-deficient BPZE1 mutant while the pertussis-specific antibody profile and Th17 response were comparable to those observed in BPZE1-immunized mice. Our findings thus support a role of CyaA in BPZE1-mediated protection through induction of cellular mediated immunity. © 2013 Institut Pasteur.|
|Source Title:||Microbes and Infection|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 19, 2018
WEB OF SCIENCETM
checked on Sep 3, 2018
checked on May 10, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.