Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.1210273109
Title: IgG1 + ovalbumin-specific B-cell transnuclear mice show class switch recombination in rare allelically included B cells
Authors: Dougan, S.K.
Ogata, S.
Hu, C.-C.A.
Grotenbreg, G.M. 
Guillen, E.
Jaenisch, R.
Ploegh, H.L.
Keywords: Allelic exclusion
Natural antibodies
TN mice
Issue Date: 21-Aug-2012
Citation: Dougan, S.K., Ogata, S., Hu, C.-C.A., Grotenbreg, G.M., Guillen, E., Jaenisch, R., Ploegh, H.L. (2012-08-21). IgG1 + ovalbumin-specific B-cell transnuclear mice show class switch recombination in rare allelically included B cells. Proceedings of the National Academy of Sciences of the United States of America 109 (34) : 13739-13744. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.1210273109
Abstract: We used somatic cell nuclear transfer (SCNT) to generate a mouse from the nucleus of an IgG1 + ovalbumin-specific B cell. The resulting OBI mice show generally normal B-cell development, with elevated percentages of marginal zone B cells and a reduction in B-1 B cells. Whereas OBI RAG1 -/- mice have exclusively IgG1 anti-ovalbumin in their serum, OBI mice show elevated levels of anti-ovalbumin of nearly all isotypes 3′ of the γ1 constant region in the IgH locus, indicating that class switch recombination (CSR) occurs in the absence of immunization with ovalbumin. This CSR is associated with the presence of IgM +IgG1 + double producer B cells that represent
Source Title: Proceedings of the National Academy of Sciences of the United States of America
URI: http://scholarbank.nus.edu.sg/handle/10635/126727
ISSN: 00278424
DOI: 10.1073/pnas.1210273109
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