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|Title:||IgG1 + ovalbumin-specific B-cell transnuclear mice show class switch recombination in rare allelically included B cells|
|Citation:||Dougan, S.K., Ogata, S., Hu, C.-C.A., Grotenbreg, G.M., Guillen, E., Jaenisch, R., Ploegh, H.L. (2012-08-21). IgG1 + ovalbumin-specific B-cell transnuclear mice show class switch recombination in rare allelically included B cells. Proceedings of the National Academy of Sciences of the United States of America 109 (34) : 13739-13744. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.1210273109|
|Abstract:||We used somatic cell nuclear transfer (SCNT) to generate a mouse from the nucleus of an IgG1 + ovalbumin-specific B cell. The resulting OBI mice show generally normal B-cell development, with elevated percentages of marginal zone B cells and a reduction in B-1 B cells. Whereas OBI RAG1 -/- mice have exclusively IgG1 anti-ovalbumin in their serum, OBI mice show elevated levels of anti-ovalbumin of nearly all isotypes 3′ of the γ1 constant region in the IgH locus, indicating that class switch recombination (CSR) occurs in the absence of immunization with ovalbumin. This CSR is associated with the presence of IgM +IgG1 + double producer B cells that represent|
|Source Title:||Proceedings of the National Academy of Sciences of the United States of America|
|Appears in Collections:||Staff Publications|
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