Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.joen.2011.06.017
Title: Role of efflux pump inhibitors on the antibiofilm efficacy of calcium hydroxide, chitosan nanoparticles, and light-activated disinfection
Authors: Upadya, M. 
Shrestha, A.
Kishen, A.
Keywords: Biofilm
calcium hydroxide
efflux
Enterococcus faecalis
light activated disinfection
nanoparticles
Issue Date: Oct-2011
Citation: Upadya, M., Shrestha, A., Kishen, A. (2011-10). Role of efflux pump inhibitors on the antibiofilm efficacy of calcium hydroxide, chitosan nanoparticles, and light-activated disinfection. Journal of Endodontics 37 (10) : 1422-1426. ScholarBank@NUS Repository. https://doi.org/10.1016/j.joen.2011.06.017
Abstract: Introduction: The purpose of this study was to evaluate the role of efflux pumps in altering the susceptibility of Enterococcus faecalis biofilms to calcium hydroxide (Ca(OH) 2), chitosan nanoparticles, and light-activated disinfection (LAD). Methods: E. faecalis as 4-day-old biofilms and biofilm-derived cells were tested with aqueous Ca(OH) 2 in concentrations of 25%, 50%, and 100%; chitosan nanoparticles in concentrations of 10 and 20 mg/mL (3, 12, and 24 hours); and methylene blue (MB) mediated LAD at an energy dose range of 2-40 J/cm 2. An efflux pump inhibitor (EPI) was incorporated into all 3 modalities of treatment. The antimicrobial activity was assessed by determining the colony-forming units. Results: E. faecalis biofilms, in contrast to the biofilm-derived cells, were found to persist even after 24-hour treatment with different concentrations of Ca(OH) 2 and chitosan nanoparticles. LAD at an energy dose of 40 J/cm 2 completely inactivated 4-day-old E. faecalis biofilms. The addition of EPI improved the antibiofilm efficacy of Ca(OH) 2 at lower concentrations (P
Source Title: Journal of Endodontics
URI: http://scholarbank.nus.edu.sg/handle/10635/125515
ISSN: 00992399
DOI: 10.1016/j.joen.2011.06.017
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