Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0054862
Title: Cyclic AMP Responsive Element Binding Proteins Are Involved in 'Emergency' Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β
Authors: Hirai, H.
Kamio, N.
Huang, G.
Matsusue, A.
Ogino, S.
Kimura, N.
Satake, S.
Ashihara, E.
Imanishi, J.
Tenen, D.G. 
Maekawa, T.
Issue Date: 30-Jan-2013
Citation: Hirai, H., Kamio, N., Huang, G., Matsusue, A., Ogino, S., Kimura, N., Satake, S., Ashihara, E., Imanishi, J., Tenen, D.G., Maekawa, T. (2013-01-30). Cyclic AMP Responsive Element Binding Proteins Are Involved in 'Emergency' Granulopoiesis through the Upregulation of CCAAT/Enhancer Binding Protein β. PLoS ONE 8 (1) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0054862
Abstract: In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-based reporter system was developed to elucidate the molecular switch required for C/EBPβ-dependency. The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced differentiation of bone marrow cells. In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. Retroviral transduction of a dominant negative CREB mutant reduced C/EBPβ mRNA levels and significantly impaired the proliferation/differentiation of granulocyte precursors, while a constitutively active form of CREB facilitated C/EBPβ transcription. These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation. © 2013 Hirai et al.
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/125377
ISSN: 19326203
DOI: 10.1371/journal.pone.0054862
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