Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/125237
Title: REGULATION OF FIBROBLAST PROLIFERATION AND GENE EXPRESSION: IMPLICATIONS FOR OCULAR SURFACE FIBROSIS, SYSTEMIC INFLAMMATORY AND NEOPLASTIC DISEASE
Authors: LAN WANWEN
Keywords: pterygium, fibrovascular proliferation, cell cycle, microRNA, lincRNA
Issue Date: 14-Aug-2015
Citation: LAN WANWEN (2015-08-14). REGULATION OF FIBROBLAST PROLIFERATION AND GENE EXPRESSION: IMPLICATIONS FOR OCULAR SURFACE FIBROSIS, SYSTEMIC INFLAMMATORY AND NEOPLASTIC DISEASE. ScholarBank@NUS Repository.
Abstract: Pterygium is a fibrovascular proliferative disease of the ocular surface that has been linked to fibroblast proliferation and dysfunction. In my studies, profiling using microarrays found that among other significantly dysregulated non-coding RNAs, microRNA-215 and a lincRNA, linc-9432 (probe ID: A_19_P00809432) especially hold promise for further study. I showed that miR-215 was down-regulated in pterygium, and regulated fibroblast proliferation at G1 and G2 cell cycle phases through inhibition of cyclins and cell cycle-related transcripts. I validated expression levels of 24 lincRNAs found to be dysregulated in pterygium and showed that some of these have impacts on fibroblast function. Of particular interest was linc-9432, a previously unreported lincRNA, found to be down-regulated in pterygium tissue. I delineated the full sequence of linc-9432 and also suggested that it was involved in cell cycle G1 progression. These results are promising and warrant further investigation of epigenetic regulators in pterygium and other fibroblastic tissues.
URI: http://scholarbank.nus.edu.sg/handle/10635/125237
Appears in Collections:Ph.D Theses (Open)

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