Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/124178
Title: HEPATOCYTE POLARITY AND FUNCTION ENHANCEMENT THROUGH SCALABLE COMPACTION
Authors: ZHU LIANG
Keywords: Compaction, vertical-flow perfusion, scalable, bioreactor, hepatocyte, drug testing
Issue Date: 21-Jan-2016
Citation: ZHU LIANG (2016-01-21). HEPATOCYTE POLARITY AND FUNCTION ENHANCEMENT THROUGH SCALABLE COMPACTION. ScholarBank@NUS Repository.
Abstract: This thesis applied mechanobiology principles to scale up a microfluidic proof-of-concept culture device into high throughput bioreactors by incorporating compaction in perfusion culture. The Scalable Compaction Bioreactor Array (SCBA) uses PMMA to replace the PDMS in order to facilitate potential mass production. A first-of-its-kind ?vertical flow? design has been adopted to generate and control compaction in large culture area in the multi-well array. Hepatocytes, an important cell type utilized in drug toxicology testing platforms, were maintained with enhanced hepatic functions and polarities over a long term. Notably, cytoskeleton actin was found in distributions similar to in vivo tissue without support of ECM scaffold or overlays, which enables 3D culture without adsorption by supporting materials and facilitate drug testing applications.
URI: http://scholarbank.nus.edu.sg/handle/10635/124178
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