DEVELOPING NOVEL CHEMICAL BIOLOGY TOOLS FOR INTERROGATION OF CELLULAR DRUG TARGETS AT MOLECULAR AND CELLULAR LEVELS

Abstract

IN CHEMICAL BIOLOGY, THERE ARE MANY TOOLS WIDELY USED FOR CELL IMAGING, TARGET/INHIBITOR IDENTIFICATION, TARGET SCREENING AND OTHER FUNCTIONS, SUCH AS CHEMICAL PROBES OR MICROARRAY TECHNOLOGY. CHEMICAL PROBES ARE MAINLY FUNCTIONED IN PROTEOME STUDY AND BIO-IMAGING, MOST OF WHICH ARE SELECTIVE AND ACCURATE AT CELLULAR LEVELS; HOWEVER, MICROARRAY IS MAINLY USED IN HIGH THROUGHPUT TARGET SCREENING AND FOLLOWED BY TARGET IDENTIFICATION PROCESSES, WHICH WAS HIGHLY EFFICIENT. IN THIS STUDY, THE DEVELOPMENT OF NOVEL CHEMICAL BIOLOGY TOOLS FOR INTERROGATION OF CELLULAR DRUG TARGETS AT MOLECULAR AND CELLULAR LEVELS WAS MAINLY DISCUSSED; INCLUDING THE DEVELOPMENT OF NEWLY DESIGNED ACTIVITY-BASED PROBES AND AFFINITY-BASED PROBES, AS WELL AS THE NEW APPLICATIONS OF SMALL MOLECULE ARRAY AND CELL-BASED MICROARRAY TECHNOLOGY.