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Title: | THE DEPENDENCY OF FAITHFUL CELL DIVISION ON HYPERPHOSPHORYLATION OF ACUTE MYELOID LEUKAEMIA ASSOCIATED CYTOPLASMIC NUCLEOPHOSMIN | Authors: | CHAN MEI YI NARISA | Keywords: | NPMc, AML, phosphorylation, centrosome, mitosis, nuclear-cytoplasm | Issue Date: | 22-Jul-2015 | Citation: | CHAN MEI YI NARISA (2015-07-22). THE DEPENDENCY OF FAITHFUL CELL DIVISION ON HYPERPHOSPHORYLATION OF ACUTE MYELOID LEUKAEMIA ASSOCIATED CYTOPLASMIC NUCLEOPHOSMIN. ScholarBank@NUS Repository. | Abstract: | Nucleophosmin (NPM) is a multifunctional protein that is predominantly localised in the nucleolus&46; In one-third of acute myeloid leukaemia (AML), a mutation in the C-terminus causes cytoplasmic delocalisation&46; This mutant, known as NPMc, doesn&39;t contribute to leukaemogenesis by upregulating XIAP and cannot inhibit caspases 3 and 9&46; Instead, centrosome-related phosphorylation sites threonine 199 and serine 4 are hyperphosphorylated on NPMc which is of interest as 85% of NPMc&43;AML have normal karyotype upon which normal centrosome duplication is dependant&46; Kinase and phosphatase levels do not differ between wtNPM and NPMc-expressing cells and phosphorylation decreases upon nuclear relocalisation&46; Furthermore, G2&47;M phase centrosome phosphorylation sites are not differentially phosphorylated&46; Inhibition of phosphorylation leads to cell cycle arrest in G2&47;M phase&46; This work provides evidence that normal karyotype in NPMc&43;AML is linked to centrosome-related phosphorylation of NPM paving the way for targeting NPMc in conjunction with current chemotherapeutics to enhance DNA-damaging and treatment prognosis&46; | URI: | http://scholarbank.nus.edu.sg/handle/10635/121938 |
Appears in Collections: | Ph.D Theses (Open) |
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