Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121938
Title: THE DEPENDENCY OF FAITHFUL CELL DIVISION ON HYPERPHOSPHORYLATION OF ACUTE MYELOID LEUKAEMIA ASSOCIATED CYTOPLASMIC NUCLEOPHOSMIN
Authors: CHAN MEI YI NARISA
Keywords: NPMc, AML, phosphorylation, centrosome, mitosis, nuclear-cytoplasm
Issue Date: 22-Jul-2015
Source: CHAN MEI YI NARISA (2015-07-22). THE DEPENDENCY OF FAITHFUL CELL DIVISION ON HYPERPHOSPHORYLATION OF ACUTE MYELOID LEUKAEMIA ASSOCIATED CYTOPLASMIC NUCLEOPHOSMIN. ScholarBank@NUS Repository.
Abstract: Nucleophosmin (NPM) is a multifunctional protein that is predominantly localised in the nucleolus&46; In one-third of acute myeloid leukaemia (AML), a mutation in the C-terminus causes cytoplasmic delocalisation&46; This mutant, known as NPMc, doesn&39;t contribute to leukaemogenesis by upregulating XIAP and cannot inhibit caspases 3 and 9&46; Instead, centrosome-related phosphorylation sites threonine 199 and serine 4 are hyperphosphorylated on NPMc which is of interest as 85% of NPMc&43;AML have normal karyotype upon which normal centrosome duplication is dependant&46; Kinase and phosphatase levels do not differ between wtNPM and NPMc-expressing cells and phosphorylation decreases upon nuclear relocalisation&46; Furthermore, G2&47;M phase centrosome phosphorylation sites are not differentially phosphorylated&46; Inhibition of phosphorylation leads to cell cycle arrest in G2&47;M phase&46; This work provides evidence that normal karyotype in NPMc&43;AML is linked to centrosome-related phosphorylation of NPM paving the way for targeting NPMc in conjunction with current chemotherapeutics to enhance DNA-damaging and treatment prognosis&46;
URI: http://scholarbank.nus.edu.sg/handle/10635/121938
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