Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121755
Title: DEVELOPMENT AND BIOLOGICAL APPLICATIONS OF HIGH-RESOLUTION ION BEAM INDUCED FLUORESCENCE MICROSCOPY
Authors: MI ZHAOHONG
Keywords: Super-resolution imaging, MeV ion beam microscopy, ion-beam-induced fluorescence imaging, STIM, lanthanide-doped nanocrystals, subcellular bioimaging
Issue Date: 20-Aug-2015
Citation: MI ZHAOHONG (2015-08-20). DEVELOPMENT AND BIOLOGICAL APPLICATIONS OF HIGH-RESOLUTION ION BEAM INDUCED FLUORESCENCE MICROSCOPY. ScholarBank@NUS Repository.
Abstract: Subcellular imaging through specific fluorescence labeling of cellular components plays an indispensable role in biological and biomedical studies. Among those fluorescence imaging techniques, mega-electron-volt (MeV) ion-induced fluorescence microscopy has unique advantages because MeV ions can penetrate through biological cells with little deflection in their trajectories. In this thesis, the primary aim is to develop the ion beam induced fluorescence technique for high-resolution imaging of biological specimens. To achieve this goal, an efficient light collection and detection system through employing a customized parabolic mirror, appropriate fluorescent probes (NaYF4:Yb/Tm nanocrystals) without distinct iono-bleaching, as well as new sample preparation protocols were developed. An additional feature using the doping-concentration-optimized nanoparticles NaYF4:Yb/Tm (60/2 mol%) as the fluorescent probes is that structural and fluorescence imaging of a whole cell using scanning transmission ion microscopy (STIM) and Ion Beam Induced Fluorescence Imaging at sub 100 nm resolutions can be achieved simultaneously.
URI: http://scholarbank.nus.edu.sg/handle/10635/121755
Appears in Collections:Ph.D Theses (Open)

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