Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121748
Title: STRUCTURE AND ASSEMBLY OF SINGAPORE GROUPER INDOVIRUS
Authors: LIU YANG
Keywords: SGIV; structure; assembly; ORF075R; TEM; tomography
Issue Date: 24-Jun-2015
Source: LIU YANG (2015-06-24). STRUCTURE AND ASSEMBLY OF SINGAPORE GROUPER INDOVIRUS. ScholarBank@NUS Repository.
Abstract: Singapore grouper iridovirus (SGIV) is a typical large DNA virus. And we have treated this virus as a model and extensively studied it for the past decade. Here we use advanced TEM techniques to study the viral life cycle of SGIV. Different assembly intermediates were observed at different stages of viral infection. And the Vesicle-tubule Liked Structures were found and considered as a key player in the budding process during viral life cycle. We also performed a functional analysis of ORF075R, an important structural protein of SGIV. We found that ORF075R was located in the electron dense middle layer of SGIV, i.e., between the inner envelope layer of the capsid shell and the DNA core. Knockdown of ORF075R resulted in the forming of abnormal virion particles with defected middle layer and core. All these findings above revealed that ORF075R might be a key player during the viral assembly process.
URI: http://scholarbank.nus.edu.sg/handle/10635/121748
Appears in Collections:Ph.D Theses (Open)

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