Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121642
Title: CYCLICAL REGULATION OF A CONTRACTILE TUBE: INSIGHTS FROM THE CAENORHABDITIS ELEGANS SPERMATHECA
Authors: TAN PEI YI
Keywords: C. elegans, spermatheca, contractility, SPV-1, mechanotransduction, epithelial tube
Issue Date: 20-Aug-2015
Citation: TAN PEI YI (2015-08-20). CYCLICAL REGULATION OF A CONTRACTILE TUBE: INSIGHTS FROM THE CAENORHABDITIS ELEGANS SPERMATHECA. ScholarBank@NUS Repository.
Abstract: ACTOMYOSIN CONTRACTILITY IS A MAJOR FORCE DRIVING CHANGES IN TISSUE SHAPE. BIOCHEMICAL SIGNALING PATHWAYS DOWNSTREAM OF RHOA CONTROL ACTIN ASSEMBLY AND NON-MUSCLE MYOSIN II PHOSPHORYLATION, THUS PLAYING AN IMPORTANT ROLE IN REGULATING ACTOMYOSIN CONTRACTILITY. HOWEVER, THE MECHANOTRANSDUCTION PATHWAY FROM TISSUE STRETCHING TO RHOA ACTIVATION IS POORLY UNDERSTOOD. HERE WE SHOW THAT IN C. ELEGANS OVULATION THE F-BAR AND RHOGAP PROTEIN SPV-1 FUNCTIONS TO TRANSDUCE PHYSICAL CUES FROM THE MEMBRANE INTO A BIOCHEMICAL SIGNAL CONTROLLING CONTRACTILITY. SPV-1 LOCALIZES THROUGH ITS F-BAR DOMAIN TO THE APICAL MEMBRANE OF THE RELAXED SPERMATHECA, WHERE IT INHIBITS RHOA ACTIVITY THROUGH ITS RHOGAP DOMAIN. OOCYTE ENTRY FORCES THE SPERMATHECA CELLS TO STRETCH AND SPV-1 DETACHES FROM THE MEMBRANE PERMITTING RHOA ACTIVITY TO INCREASE. THE INCREASE IN RHOA ACTIVITY FACILITATES SPERMATHECA CONTRACTION AND EXPULSION OF THE NEWLY FERTILIZED EMBRYO INTO THE UTERUS. OUR RESULTS DEMONSTRATE HOW MEMBRANE CURV
URI: http://scholarbank.nus.edu.sg/handle/10635/121642
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
TanPY.pdf3.27 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

78
checked on Oct 12, 2018

Download(s)

20
checked on Oct 12, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.