Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121542
Title: ENGINEERING OF RECOMBINANT WHOLE-CELL BIOCATALYSTS FOR GREEN AND EFFICIENT PRODUCTION OF CHIRAL CHEMICALS
Authors: WU SHUKE
Keywords: Biocatalysis, Cascade Biocatalysis, Enantioselectivity, Enzyme, Chiral Chemicals, Alkene Functionalization
Issue Date: 14-Jan-2015
Citation: WU SHUKE (2015-01-14). ENGINEERING OF RECOMBINANT WHOLE-CELL BIOCATALYSTS FOR GREEN AND EFFICIENT PRODUCTION OF CHIRAL CHEMICALS. ScholarBank@NUS Repository.
Abstract: BIOCATALYSIS IS A GREEN AND USEFUL TOOL FOR CHEMICAL PRODUCTION, ESPECIALLY FOR THE PRODUCTION OF ENANTIOPURE FINE CHEMICALS AND PHARMACEUTICAL INTERMEDIATES, WITH WIDE INDUSTRIAL APPLICATION AND HUGE MARKET. NEVERTHELESS, MANY BIOCATALYTIC REACTIONS ARE STILL NOT EFFICIENT FOR PRACTICAL APPLICATION, AND MOST OF INDUSTRIAL BIOTRANSFORMATIONS ARE MAINLY BASED ON ONE-STEP BIOCATALYSIS. TO ADDRESS SOME OF THESE PROBLEMS IN THIS PH. D. THESIS, WE ENGINEERED A RECOMBINANT WHOLE-CELL BIOCATALYST EXPRESSING A NEWLY IDENTIFIED EPOXIDE HYDROLASE FOR ENANTIOSELECTIVE HYDROLYSIS TO PRACTICALLY PRODUCE SEVERAL IMPORTANT ENANTIOPURE EPOXIDES AND VICINAL DIOLS, DEVELOPED NOVEL WHOLE-CELL BASED CASCADE BIOCATALYSIS FOR ASYMMETRIC DIHYDROXYLATION OF ALKENES TO EFFICIENTLY PREPARE BOTH ENANTIOMERS OF THE CORRESPONDING VICINAL DIOLS IN HIGH EE AND YIELD, AND DEVELOPED NOVEL AND EFFICIENT MODULAR CASCADE BIOCATALYSIS TO ACHIEVE ONE-POT MULTI-STEP ASYMMETRIC OXY- AND AMINO-FUNCTIONALIZATION OF ARYL ALKENE
URI: http://scholarbank.nus.edu.sg/handle/10635/121542
Appears in Collections:Ph.D Theses (Open)

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