Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/121530
Title: MODULATION OF C-DI-GMP LEVELS REGULATE VIRULENCE IN PSEUDOMONAS AERUGINOSA
Authors: CHUA SONG LIN
Keywords: Cyclic-di-GMP, Biofilms, Pseudomonas aeruginosa, dispersal, host-microbe interactions, antibiotic resistance
Issue Date: 26-May-2015
Citation: CHUA SONG LIN (2015-05-26). MODULATION OF C-DI-GMP LEVELS REGULATE VIRULENCE IN PSEUDOMONAS AERUGINOSA. ScholarBank@NUS Repository.
Abstract: ACUTE AND CHRONIC INFECTIONS CAUSED BY PATHOGENIC BACTERIA, ARE TYPICALLY MEDIATED BY THE PLANKTONIC AND BIOFILM (MULTICELLULAR COMMUNITIES) LIFESTYLES RESPECTIVELY. THE ROLES OF BIOFILM FORMATION IN INFECTIONS ARE WELL-STUDIED, BUT THE BIOFILM DISPERSAL FOR DISSEMINATION OF INFECTIONS AND INDUCTION OF VIRULENCE AGAINST IMMUNE SYSTEMS, REMAINS LARGELY UNKNOWN. HERE, DISPERSED CELLS FROM PSEUDOMONAS AERUGINOSA BIOFILMS WERE FIRST SHOWN TO POSSESS A UNIQUE TRANSCRIPTOME, AS COMPARED TO PLANKTONIC AND BIOFILM CELLS. DISPERSED CELLS DISPLAYED INCREASED VIRULENCE AGAINST MURINE MACROPHAGES AND CAENORHABDITIS ELEGANS, BUT WERE HIGHLY SENSITIVE TO IRON STRESS. NEXT, BIOFILMS WERE ABLE TO BE DISPERSED BY NITRIC OXIDE (NO), A KILLING FACTOR PRODUCED BY THE MACROPHAGES. THIS RESULTED IN THE PRODUCTION OF CYTOTOXIC RHAMNOLIPIDS BY THE BACTERIAL CELLS VIA SMALL RNA SIGNALLING, TO KILL MACROPHAGES. THESE RESULTS SHED NEW LIGHT THAT BIOFILMS COULD DISPERSE AND ELICIT VIRULENCE AGAINST IMMUNE CELL
URI: http://scholarbank.nus.edu.sg/handle/10635/121530
Appears in Collections:Ph.D Theses (Open)

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